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P24928 Proteins with OtherRepeats Repeats
Uniprot ID: P24928
Protein name : DNA-directed RNA polymerase II subunit RPB1
Gene : POLR2A POLR2
Protein Families : RNA polymerase beta' chain family
Squence Length : 1970
Sequence
>P24928 1971 MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPASPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN
Repeat regions
REPEAT 1593 1599 1 REPEAT 1600 1606 2 approximate REPEAT 1608 1614 3 REPEAT 1615 1621 4 REPEAT 1622 1628 5 REPEAT 1629 1635 6 REPEAT 1636 1642 7 REPEAT 1643 1649 8 REPEAT 1650 1656 9 REPEAT 1657 1663 10 REPEAT 1664 1670 11 REPEAT 1671 1677 12 REPEAT 1678 1684 13 REPEAT 1685 1691 14 REPEAT 1692 1698 15 REPEAT 1699 1705 16 REPEAT 1706 1712 17 REPEAT 1713 1719 18 REPEAT 1720 1726 19 REPEAT 1727 1733 20 REPEAT 1734 1740 21 REPEAT 1741 1747 22 REPEAT 1748 1754 23 REPEAT 1755 1761 24 REPEAT 1762 1768 25 REPEAT 1769 1775 26 REPEAT 1776 1782 27 REPEAT 1783 1789 28 REPEAT 1790 1796 29 REPEAT 1797 1803 30 REPEAT 1804 1810 31 REPEAT 1811 1817 32 REPEAT 1818 1824 33 REPEAT 1825 1831 34 REPEAT 1832 1838 35 REPEAT 1839 1845 36 REPEAT 1846 1852 37 REPEAT 1853 1859 38 REPEAT 1860 1866 39 REPEAT 1867 1873 40 REPEAT 1874 1880 41 REPEAT 1881 1887 42 REPEAT 1888 1894 43 REPEAT 1895 1901 44 REPEAT 1902 1908 45 REPEAT 1909 1915 46 REPEAT 1916 1922 47 REPEAT 1923 1929 48 REPEAT 1930 1936 49 REPEAT 1940 1946 50 REPEAT 1947 1953 51 approximate REPEAT 1954 1960 52 approximate
Sequence region of repeats
1593- 1599 PTSPAYE 1600- 1606 PRSPGGY - H 1608- 1614 PQSPSYS 1615- 1621 PTSPSYS 1622- 1628 PTSPSYS 1629- 1635 PTSPNYS 1636- 1642 PTSPSYS 1643- 1649 PTSPSYS 1650- 1656 PTSPSYS 1657- 1663 PTSPSYS 1664- 1670 PTSPSYS 1671- 1677 PTSPSYS 1678- 1684 PTSPSYS 1685- 1691 PTSPSYS 1692- 1698 PTSPSYS 1699- 1705 PTSPSYS 1706- 1712 PTSPSYS 1713- 1719 PTSPSYS 1720- 1726 PTSPSYS 1727- 1733 PTSPSYS 1734- 1740 PTSPSYS 1741- 1747 PTSPNYS 1748- 1754 PTSPNYT 1755- 1761 PTSPSYS 1762- 1768 PTSPSYS 1769- 1775 PTSPNYT 1776- 1782 PTSPNYS 1783- 1789 PTSPSYS 1790- 1796 PTSPSYS 1797- 1803 PTSPSYS 1804- 1810 PSSPRYT 1811- 1817 PQSPTYT 1818- 1824 PSSPSYS 1825- 1831 PSSPSYS 1832- 1838 PASPKYT 1839- 1845 PTSPSYS 1846- 1852 PSSPEYT 1853- 1859 PTSPKYS 1860- 1866 PTSPKYS 1867- 1873 PTSPKYS 1874- 1880 PTSPTYS 1881- 1887 PTTPKYS 1888- 1894 PTSPTYS 1895- 1901 PTSPVYT 1902- 1908 PTSPKYS 1909- 1915 PTSPTYS 1916- 1922 PTSPKYS 1923- 1929 PTSPTYS 1930- 1936 PTSPKGS 1940- 1946 PTSPGYS 1947- 1953 PTSPTYS - H 1954- 1960 LTSPAIS - H
Function
"DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity).; (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome"
Mutation
1810 1810 R->A: Misexpression of a variety of small nuclear RNAs and small nucleolar RNAs 1838 1838 K->R: Loss of acetylation and loss of regulation of growth-factor-induced gene expression when associated with R-1859 R-1866 R-1873 R-1887 R-1908 R-1922 and R-1936 1859 1859 K->R: Loss of acetylation and loss of regulation of growth-factor-induced gene expression when associated with R-1838 R-1866 R-1873 R-1887 R-1908 R-1922 and R-1936 1866 1866 K->R: Loss of acetylation and loss of regulation of growth-factor-induced gene expression when associated with R-1859 R-1859 R-1873 R-1887 R-1908 R-1922 and R-1936 1873 1873 K->R: Loss of acetylation and loss of regulation of growth-factor-induced gene expression when associated with R-1838 R-1859 R-1866 R-1887 R-1908 R-1922 and R-1936 1887 1887 K->R: Loss of acetylation and loss of regulation of growth-factor-induced gene expression when associated with R-1838 R-1859 R-1866 R-1873 R-1908 R-1922 and R-1936 1908 1908 K->R: Loss of acetylation and loss of regulation of growth-factor-induced gene expression when associated with R R-1859 R-1866 R-1873 R-1887 R-1922 and R-1936 1922 1922 K->R: Loss of acetylation and loss of regulation of growth-factor-induced gene expression when associated with R-1838 R-1859 R-1866 R-1873 R-1887 R-1908 and R-1936 1936 1936 K->R: Loss of acetylation and loss of regulation of growth-factor-induced gene expression when associated with R-1838 R-1859 R-1866 R-1873 R-1887 R-1908 and R-1922