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O00308 Proteins WW domain Repeats
Uniprot ID:O00308
Protein name: NEDD4-like E3 ubiquitin-protein ligase WWP2
Gene : WWP2
Protein Family:
Squence Length : 870
Sequnce
>O00308 871 MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIILNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENMQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGNVPNGSALTDGSQLPSRDSSGTAVAPENRHQPPSTNCFGGRSRTHRHSGASARTTPATGEQSPGARSRHRQPVKNSGHSGLANGTVNDEPTTATDPEEPSVVGVTSPPAAPLSVTPNPNTTSLPAPATPAEGEEPSTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE
Domains
DOMAIN 20 100 C2 DOMAIN 300 333 WW 1 DOMAIN 330 363 WW 2 DOMAIN 405 437 WW 3 DOMAIN 444 477 WW 4 DOMAIN 536 870 HECT
WW sequence regions
300 - 333 LPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPG 330 - 363 LPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEY 405 - 437 LPPGWEKRQDNGRVYYVNHNTRTTQWEDPRTQG 444 - 477 LPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGF
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation
Active Site
ACT_SITE 838 838 Glycyl thioester intermediate
Mutation
498 498 K->R: Does not affect FBXL15-mediated ubiquitination 500 500 H->K: Does not affect FBXL15-mediated ubiquitination 838 838 C->A: Abolishes ubiquitination of POU5F1