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P52597 Proteins RRM domain Repeats
Uniprot ID:P52597
Protein name: Heterogeneous nuclear ribonucleoprotein F
Gene : HNRNPF HNRPF
Protein Family:
Squence Length : 415
Sequnce
>P52597 416 MMLGPEGGEGFVVKLRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPYDRPGTARRYIGIVKQAGLERMRPGAYSTGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSEFTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD
Domains
DOMAIN 13 85 RRM 1 DOMAIN 111 188 RRM 2 DOMAIN 289 366 RRM 3
RRM sequence regions
13 - 85 LRGLPWSCSVEDVQNFLSDCTIHDGAAGVHFIYTREGRQSGEAFVELGSEDDVKMALKKDRESMGHRYIEVFK 111 - 188 VRLRGLPFGCTKEEIVQFFSGLEIVPNGITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEE 289 - 366 VHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRANMQHRYIELFLNSTTGAS
Function
"Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state"
Mutation
20 20 W->A: Loss of RNA-binding 84 84 E->A: Loss of RNA-binding 116 116 R->A: Decreases affinity for RNA oligonucleotide 100-fold 120 120 F->A: Little disruption of binding RNA when associated with A-180 150 150 K->A: No effect on affinity for RNA oligonucleotide 156 156 F->A: Drastically effects folding of RRM2 173 173 K->A: Minimal effect on affinity for RNA oligonucleotide 178 178 H->A: Little disruption of binding RNA 179 179 R->A: Decreases affinity for RNA oligonucleotide 100-fold 180 180 Y->A: Decreases affinity for RNA oligonucleotide 10-fold when associated with A-120 182 182 E->A: Decreases affinity for RNA oligonucleotide 100-fold 184 184 F->A: Minimal effect on affinity for RNA oligonucleotide