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P54619 Proteins CBS domain Repeats

Uniprot ID:P54619	Protein name: 5'-AMP-activated protein kinase subunit gamma-1	Gene : PRKAG1
Protein Family:5'-AMP-activated protein kinase gamma subunit family	Squence Length : 331

Sequnce	>P54619 332 METVISSDSSPAVENEHPQETPESNNSVYTSFMKSHRCYDLIPTSSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKKP
Domains	DOMAIN 43 103 CBS 1 DOMAIN 125 187 CBS 2 DOMAIN 198 260 CBS 3 DOMAIN 272 329 CBS 4
CBS sequence regions	43 - 103 SSKLVVFDTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQ 125 - 187 KPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFM 198 - 260 TYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSV 272 - 329 EGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKKP
Function	"AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive"
Motifs	MOTIF 138 159 AMPK pseudosubstrate
Binding Site	"BINDING 70 70 AMP, ADP or ATP 2. BINDING 130 130 AMP, ADP or ATP 1. via amide nitrogen and carbonyl oxygen. BINDING 151 151 AMP 3. BINDING 170 170 AMP, ADP or ATP 2. BINDING 200 200 AMP 3. BINDING 205 205 AMP 3. via amide nitrogen and carbonyl oxygen. BINDING 269 269 AMP, ADP or ATP 2. BINDING 277 277 AMP, ADP or ATP 2. via amide nitrogen and carbonyl oxygen. BINDING 298 298 AMP 3"
Nucleotide Binding	"NP_BIND 87 90 AMP, ADP or ATP 1. NP_BIND 151 152 AMP, ADP or ATP 1. NP_BIND 226 227 AMP 3. NP_BIND 242 245 AMP, ADP or ATP 2. NP_BIND 298 299 AMP, ADP or ATP 2. NP_BIND 314 317 AMP 3"
Mutation	90 90 D->A: Reduced AMP-activation of phosphorylation of PRKAA1 or PRKAA2 245 245 D->A: Reduced AMP-activation of phosphorylation of PRKAA1 or PRKAA2 317 317 D->A: Reduced AMP-activation of phosphorylation of PRKAA1 or PRKAA2