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Q8WTX7 Proteins ACT domain Repeats
Uniprot ID:Q8WTX7
Protein name: Cytosolic arginine sensor for mTORC1 subunit 1
Gene : CASTOR1 GATSL3
Protein Family:GATS family
Squence Length : 329
Sequnce
>Q8WTX7 330 MELHILEHRVRVLSVARPGLWLYTHPLIKLLFLPRRSRCKFFSLTETPEDYTLMVDEEGFKELPPSEFLQVAEATWLVLNVSSHSGAAVQAAGVTKIARSVIAPLAEHHVSVLMLSTYQTDFILVREQDLSVVIHTLAQEFDIYREVGGEPVPVTRDDSSNGFPRTQHGPSPTVHPIQSPQNRFCVLTLDPETLPAIATTLIDVLFYSHSTPKEAASSSPEPSSITFFAFSLIEGYISIVMDAETQKKFPSDLLLTSSSGELWRMVRIGGQPLGFDECGIVAQIAGPLAAADISAYYISTFNFDHALVPEDGIGSVIEVLQRRQEGLAS
Domains
DOMAIN 72 138 ACT 1 DOMAIN 260 321 ACT 2
ACT sequence regions
72 - 138 ATWLVLNVSSHSGAAVQAAGVTKIARSVIAPLAEHHVSVLMLSTYQTDFILVREQDLSVVIHTLAQE 260 - 321 LWRMVRIGGQPLGFDECGIVAQIAGPLAAADISAYYISTFNFDHALVPEDGIGSVIEVLQRR
Function
"Functions as an intracellular arginine sensor within the amino acid-sensing branch of the TORC1 signaling pathway. As a homodimer or a heterodimer with CASTOR2, binds and inhibits the GATOR subcomplex GATOR2 and thereby mTORC1. Binding of arginine to CASTOR1 allosterically disrupts the interaction of CASTOR1-containing dimers with GATOR2 which can in turn activate mTORC1 and the TORC1 signaling pathway"
Binding Site
BINDING 274 274 Arginine. via carbonyl oxygen
Mutation
90 90 Q->A: No effect on interaction with the GATOR2 complex 96 96 K->A: No effect on interaction with the GATOR2 complex 99 99 R->A: No effect on interaction with the GATOR2 complex 108 110 HHV->QNI: Loss of arginine-binding 111 111 S->A: Loss of arginine-binding 113 113 L->F: No effect on interaction with the GATOR2 complex 118 119 YQ->AA: No effect on arginine-binding 121 121 D->A: No effect on arginine-binding 126 126 R->A: Decreased arginine-binding 175 175 H->A: Decreased arginine-binding 202 202 I->E: No effect on arginine-binding 207 207 Y->S: No effect on arginine-binding 261 261 E->A: No effect on arginine-binding 264 264 R->A: No effect on interaction with the GATOR2 complex 276 276 D->A: Decreased arginine-binding 277 277 E->A: Decreased arginine-binding 278 278 C->A: Decreased arginine-binding 280 280 I->A: Loss of arginine-binding 292 292 D->A: No effect on arginine-binding 302 302 N->K: No effect on interaction with the GATOR2 complex 304 304 D->A: Loss of arginine-binding