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Q96RL1 Proteins UIM domain Repeats
Uniprot ID:Q96RL1
Protein name: BRCA1-A complex subunit RAP80
Gene : UIMC1 RAP80 RXRIP110
Protein Family:RAP80 family
Squence Length : 719
Sequnce
>Q96RL1 720 MPRRKKKVKEVSESRNLEKKDVETTSSVSVKRKRRLEDAFIVISDSDGEEPKEENGLQKTKTKQSNRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIAESLNSCRPSDASATRSRPLATGPSSQSHQEKTTDSGLTEGIWQLVPPSLFKGSHISQGNEAEEREEPWDHTEKTEEEPVSGSSGSWDQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQESTGRGSAFLKAVQGSGDTSRHCLPTLADAKGLQDTGGTVNYFWGIPFCPDGVDPNQYTKVILCQLEVYQKSLKMAQRQLLNKKGFGEPVLPRPPSLIQNECGQGEQASEKNECISEDMGDEDKEERQESRASDWHSKTKDFQESSIKSLKEKLLLEEEPTTSHGQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF
Domains
DOMAIN 80 99 UIM 1 DOMAIN 105 124 UIM 2
UIM sequence regions
80 - 99 EEQFALALKMSEQEAREVNS 105 - 124 EELLRKAIAESLNSCRPSDA
Function
"Ubiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.37). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.37}"
Motifs
MOTIF 60 78 LR motif
Mutation
"9 9 K->A: Does not affect symoylation when associated with A-19 A-31 A-52 and A-61 19 19 K->A: Does not affect symoylation when associated with A-9 A-31 A-52 and A-61 31 31 K->A: Does not affect symoylation when associated with A-9 A-19 A-52 and A-61 52 52 K->A: Does not affect symoylation when associated with A-9 A-19 A-31 and A-61 61 61 K->A: Does not affect symoylation when associated with A-9 A-19 A-31 and A-52 81 81 Missing: Strongly reduces ubiquitin binding via UIM 1 88 88 A->G,S: Impairs localization to DNA damages sites when associated with A-92 S-113 and A-117 92 92 S->A: Impairs localization to DNA damages sites when associated with S-88 S-113 and A-117 97 103 REVNSQE->AA: Impairs the selectivity for 'K-63'-linked ubiquitin 97 103 REVNSQE->AAAAAAA: Increases the selectivity for 'K-63'-linked ubiquitin 97 103 REVNSQE->AAAAAAAAA: Impairs the selectivity for 'K-63'-linked ubiquitin 101 101 S->A,E: Slightly impairs the selectivity for 'K-63'-linked ubiquitin 113 113 A->G,S: Impairs ubiquitin-binding and localization to DNA damages sites when associated with S-88 A-92 and A-117 117 117 S->A: Impairs ubiquitin-binding and localization to DNA damages sites when associated with S-88 A-92 and S-113 205 205 S->G: Abolishes phosphorylation at this position 508 508 C->A: Abolishes interaction with histone monoubiquitinated H2B without affecting the interaction with H2A"