S.NO	Uniprot ID	Protein Name	Sequence Length	HEAT Repeats region	Mutations	Involvement of Repeats in Mutations
1	Q14008	Cytoskeleton-associated protein 5	2032	REPEAT 159 197 HEAT 1 REPEAT 356 394 HEAT 2 REPEAT 434 472 HEAT 3 REPEAT 750 788 HEAT 4 REPEAT 855 893 HEAT 5 REPEAT 936 974 HEAT 6 REPEAT 1013 1051 HEAT 7 REPEAT 1284 1322 HEAT 8 REPEAT 1324 1357 HEAT 9 REPEAT 1361 1399 HEAT 10	1939 1939 L->A,R: Disrupts interaction with TACC3 1939 1939 L->A: Abolishes localization to spindle microtubules, no effect on localization to centrosomes and kinetochores when associated with A-1942 1942 1942 L->A,R: Disrupts interaction with TACC3 1942 1942 L->A: Abolishes localization to spindle microtubules, no effect on localization to centrosomes and kinetochores when associated with A-1939	N
2	P42345	Serine/threonine-protein kinase mTOR	2549	REPEAT 16 53 HEAT 1 REPEAT 55 99 HEAT 2 REPEAT 100 137 HEAT 3 REPEAT 138 179 HEAT 4 REPEAT 180 220 HEAT 5 REPEAT 222 276 HEAT 6 REPEAT 277 313 HEAT 7 REPEAT 314 364 HEAT 8 REPEAT 365 409 HEAT 9 REPEAT 410 445 HEAT 10 REPEAT 446 494 HEAT 11 REPEAT 495 529 HEAT 12 REPEAT 530 563 HEAT 13 REPEAT 564 596 HEAT 14 REPEAT 597 636 HEAT 15 REPEAT 637 683 HEAT 16 REPEAT 686 724 HEAT 17 REPEAT 727 766 HEAT 18 REPEAT 769 811 HEAT 19 REPEAT 814 853 HEAT 20 REPEAT 857 893 HEAT 21 REPEAT 894 942 HEAT 22 REPEAT 943 988 HEAT 23 REPEAT 989 1027 HEAT 24 REPEAT 1029 1068 HEAT 25 REPEAT 1069 1105 HEAT 26 REPEAT 1106 1144 HEAT 27 REPEAT 1145 1188 HEAT 28 REPEAT 1189 1225 HEAT 29 REPEAT 1226 1273 HEAT 30 REPEAT 1274 1311 HEAT 31 REPEAT 1312 1345 HEAT 32	2159 2159 S->A: Reduces mTORC1-associated S-2481 autophosphorylation when associated with A-2164 2159 2159 S->D: Stronger phosphorylation of RPS6KB1 when associated with E-2164 2164 2164 T->A: Reduces mTORC1-associated S-2481 autophosphorylation when associated with A-2159 2164 2164 T->E: Stronger phosphorylation of RPS6KB1 when associated with D-2159 2173 2173 T->A: Increased mTOR kinase activity 2340 2340 H->A: Barely detectable kinase activity	N
3	O75691	Small subunit processome component 20 homolog	2785	REPEAT 165 202 HEAT 1 REPEAT 1841 1878 HEAT 2	2744 2746 KKK->AAA: Inhibits nucleolar but not nuclear localization 2744 2744 K->A: Does not decrease nucleolar localization 2745 2745 K->A: Does not decrease nucleolar localization 2746 2746 K->A: Decreases nucleolar localization 2748 2749 KK->AA: Inhibits nucleolar but not nuclear localization 2748 2748 K->A: Does not decrease nucleolar localization 2749 2749 K->A: Decreases nucleolar localization 2751 2751 K->A: Does not decrease nucleolar localization 2753 2753 K->A: Does not decrease nucleolar localization 2757 2760 KKRK->AAAA: Inhibits nucleolar localization and decreases nuclear localization 2757 2757 K->A: Does not decrease nucleolar localization 2758 2758 K->A: Does not decrease nucleolar localization 2760 2760 K->A: Does not decrease nucleolar localization	N
4	O75122	CLIP-associating protein 2	1294	REPEAT 173 208 HEAT 1 REPEAT 209 245 HEAT 2 REPEAT 250 287 HEAT 3 REPEAT 710 747 HEAT 4 REPEAT 772 809 HEAT 5 REPEAT 1054 1091 HEAT 6 REPEAT 1098 1135 HEAT 7 REPEAT 1216 1253 HEAT 8	106 106 W->E: Decreases affinity for microtubules when associated with A-191 E-667 E-833 A-838 and A-839 191 191 K->A: Decreases affinity for microtubules when associated with E-106 E-667 E-833 A-838 and A-839 496 497 IP->AA: No effect on MAPRE1 binding when associated with 519-A-A-520 496 497 IP->SS: Reduced targeting to the growing microtubule plus ends when associated with 519-S-S-520 499 499 S->D: Phosphomimetic mutant that reduces MAPRE1 binding when associated with D-503 D-507 D-525 D-529 D-533 D-537 and D-541 503 503 S->D: Phosphomimetic mutant that reduces MAPRE1 binding when associated with D-499 D-507 D-525 D-529 D-533 D-537 and D-541 507 507 S->D: Phosphomimetic mutant that reduces MAPRE1 binding when associated with D-499 D-503 D-525 D-529 D-533 D-537 and D-541 519 520 IP->AA: No effect on MAPRE1 binding when associated with 496-A-A-497 519 520 IP->SS: Reduced targeting to the growing microtubule plus ends when associated with 496-S-S-497 525 525 S->D: Phosphomimetic mutant that reduces MAPRE1 binding when associated with D-499 D-503 D-507 D-529 D-533 D-537 and D-541 529 529 S->D: Phosphomimetic mutant that reduces MAPRE1 binding when associated with D-499 D-503 D-507 D-525 D-533 D-537 and D-541 533 533 S->D: Phosphomimetic mutant that reduces MAPRE1 binding when associated with D-499 D-503 D-507 D-525 D-529 D-537 and D-541 537 537 S->D: Phosphomimetic mutant that reduces MAPRE1 binding when associated with D-499 D-503 D-507 D-525 D-529 D-533 and D-541 541 541 S->D: Phosphomimetic mutant that reduces MAPRE1 binding when associated with D-499 D-503 D-507 D-525 D-529 D-533 and D-537 667 667 W->E: Decreases affinity for microtubules when associated with E-106 A-191 E-833 A-838 and A-839 833 833 K->E: Decreases affinity for microtubules when associated with E-106 A-191 E-667 A-838 and A-839 838 838 R->A: Decreases affinity for microtubules when associated with E-106 A-191 E-667 E-833 and A-839 839 839 K->A: Decreases affinity for microtubules when associated with E-106 A-191 E-667 E-833 and A-838	Y
5	Q6KC79	Nipped-B-like protein	2804	REPEAT 1767 1805 HEAT 1 REPEAT 1843 1881 HEAT 2 REPEAT 1945 1984 HEAT 3 REPEAT 2227 2267 HEAT 4 REPEAT 2313 2351 HEAT 5	1003 1003 V->A: Abolishes interaction with CBX3 when associated with A-1005 1003 1003 V->E: Abolishes interaction with CBX5 when associated with E-1005 1005 1005 L->A: Abolishes interaction with CBX3 when associated with A-1003 1005 1005 L->E: Abolishes interaction with CBX5 when associated with E-1003	N
6	Q92973	Transportin-1	898	REPEAT 19 46 HEAT 1 REPEAT 51 89 HEAT 2 REPEAT 98 131 HEAT 3 REPEAT 137 174 HEAT 4 REPEAT 181 211 HEAT 5 REPEAT 224 251 HEAT 6 REPEAT 263 290 HEAT 7 REPEAT 306 397 HEAT 8 REPEAT 405 433 HEAT 9 REPEAT 445 472 HEAT 10 REPEAT 486 519 HEAT 11 REPEAT 527 560 HEAT 12 REPEAT 568 606 HEAT 13 REPEAT 614 665 HEAT 14 REPEAT 676 707 HEAT 15 REPEAT 715 748 HEAT 16 REPEAT 756 791 HEAT 17 REPEAT 799 832 HEAT 18 REPEAT 841 872 HEAT 19 REPEAT 875 895 HEAT 20	468 468 W->A: Abolishes interaction with the ADAR nuclear localization signal 738 738 W->A: Abolishes interaction with the ADAR nuclear localization signal	Y
7	Q8IZE3	Protein-associating with the carboxyl-terminal domain of ezrin	742	REPEAT 199 238 HEAT 1 REPEAT 285 323 HEAT 2 REPEAT 333 370 HEAT 3 REPEAT 372 409 HEAT 4	2 6 GSENS->M: No Golgi targeting, accumulates in the cytoplasm	N
8	Q14974	Importin subunit beta-1	876	REPEAT 2 31 HEAT 1 REPEAT 33 65 HEAT 2 REPEAT 85 123 HEAT 3 REPEAT 129 160 HEAT 4 REPEAT 170 202 HEAT 5 REPEAT 212 247 HEAT 6 REPEAT 253 302 HEAT 7 REPEAT 314 360 HEAT 8 REPEAT 364 394 HEAT 9 REPEAT 402 438 HEAT 10 REPEAT 449 485 HEAT 11 REPEAT 500 537 HEAT 12 REPEAT 544 592 HEAT 13 REPEAT 600 639 HEAT 14 REPEAT 644 681 HEAT 15 REPEAT 686 724 HEAT 16 REPEAT 732 776 HEAT 17 REPEAT 786 829 HEAT 18 REPEAT 831 873 HEAT 19	178 178 I->A: Largely reduced binding to FxFG repeats and reduced nuclear import 178 178 I->F,D: Loss of binding to FxFG repeats and reduced nuclear import	Y
9	P42858	Huntingtin	3142	REPEAT 204 241 HEAT 1 REPEAT 246 283 HEAT 2 REPEAT 316 360 HEAT 3 REPEAT 802 839 HEAT 4 REPEAT 902 940 HEAT 5	495 495 I->A: Inhibits interaction with ZDHHC13 and ZDHHC17 498 499 QP->AA: Abolishes interaction with ZDHHC17 498 498 Q->A: Inhibits interaction with ZDHHC13 and ZDHHC17 499 499 P->A: Inhibits interaction with ZDHHC13 and ZDHHC17	N
10	Q6NUP7	Serine/threonine-protein phosphatase 4 regulatory subunit 4	873	REPEAT 213 251 HEAT 1 REPEAT 252 290 HEAT 2 REPEAT 392 427 HEAT 3	501 501 R->W: Abolishes interaction with PPP4C 618 618 V->A: Diminishes interaction with PPP4C 618 618 V->D: Abolishes interaction with PPP4C	N
11	O14980	Exportin-1	1071	REPEAT 217 240 HEAT 1 REPEAT 241 277 HEAT 2 REPEAT 354 472 HEAT 3 REPEAT 515 553 HEAT 4 REPEAT 560 597 HEAT 5 REPEAT 602 639 HEAT 6 REPEAT 775 813 HEAT 7 REPEAT 885 916 HEAT 8 REPEAT 917 954 HEAT 9 REPEAT 1002 1039 HEAT 10	191 191 S->A: Does not abolish Rex-mediated mRNA export 284 284 V->E: Does not abolish Rex-mediated mRNA export 334 334 D->G: Does not abolish Rex-mediated mRNA export 337 337 I->L: Does not abolish Rex-mediated mRNA export 346 346 T->A: Does not abolish Rex-mediated mRNA export 402 402 V->I: Does not abolish Rex-mediated mRNA export 411 411 P->T: Strongly abolishes interaction with Rex and RANBP3, abolishes Rex-mediated mRNA export when associated with S-414 when associated with S-414 412 412 M->V: Does not abolish interaction with Rex and RANBP3, and Rex-mediated mRNA export 414 414 F->S: Strongly abolishes interaction with Rex and RANBP3, abolishes Rex-mediated mRNA export when associated with T-411 when associated with T-411 428 447 EEVLVVENDQGEVVREFMKD->QQVLVVQNNQGQVVRQFMKN: Abolishes Ran binding activity in absence of cargo and abolishes partially Ran binding activity in presence of cargo 430 446 VLVVENDQGEVVREFMK->DEDEENDQGEDEEEDDD: Partially restores Ran binding activity in presence of cargo 430 433 VLVV->DEDE: Abolishes Ran binding activity both in absence or presence of cargo 454 454 Y->A: Does not abolish Ran binding activity and nuclear export complex formation 474 474 R->I: Strongly abolishes interaction with Rex and RANBP3, abolishes Rex-mediated mRNA export 481 481 H->Q: Strongly abolishes interaction with Rex and RANBP3, abolishes Rex-mediated mRNA export 513 513 E->A: Abolishes Ran binding activity and nuclear export complex formation when associated with A-553 and A-554 525 525 L->A: Enhances Ran binding activity and does not abolish nuclear export complex formation when associated with A-561 when associated with A-568 and A-572 550 550 Q->A: Enhances Ran binding activity and does not abolish nuclear export complex formation when associated with A-553 and A-590 553 553 R->A: Enhances Ran binding activity and does not abolish nuclear export complex formation when associated with A-550 and A-590 when associated with A-513 and A-554 554 554 F->A: Partially abolishes Ran binding activity and does not abolish nuclear export complex formation when associated with A-561 when associated with A-553 and A-513 561 561 F->A: Abolishes Ran binding activity and nuclear export complex formation when associated with A-554 when associated with A-525 568 568 K->A: Does not abolish Ran binding activity and partially abolish nuclear export complex formation when associated with A-525 and A-572 572 572 F->A: Does not abolish Ran binding activity and partially abolish nuclear export complex formation when associated with A-525 and A-568 583 583 M->A: Enhances Ran binding activity when associated with A-590 590 590 K->A: Enhances Ran binding activity and does not abolish nuclear export complex formation when associated with A-583 when associated with A-550 and A-553	Y
12	P78527	DNA-dependent protein kinase catalytic subunit	4128	REPEAT 288 323 HEAT 1 REPEAT 1004 1040 HEAT 2	1510 1510 L->P: Loss of interaction with C1D 1516 1517 EL->PD: Loss of interaction with C1D 2609 2609 T->A: Leads to radiation sensitivity and impaired DSB joining when associated with A-2612 2612 2612 S->A: Reduced phosphorylation when associated with A-2609 2638 2638 T->A: Alleviates phosphorylation, leaves a fully active enzyme with compromised cellular resistance to ionizing radiation without affecting DNA end joining when associated with A-2647 2647 2647 T->A: Alleviates phosphorylation, leaves a fully active enzyme with compromised cellular resistance to ionizing radiation without affecting DNA end joining when associated with A-2638	N
13	Q92797	Symplekin	1274	REPEAT 31 64 HEAT 1 REPEAT 67 101 HEAT 2 REPEAT 104 146 HEAT 3 REPEAT 153 192 HEAT 4 REPEAT 227 266 HEAT 5	185 185 K->A: Abolishes stimulation of SSU72 phosphatase activity	Y
14	Q14997	Proteasome activator complex subunit 4	1843	REPEAT 475 519 HEAT 1 REPEAT 998 1037 HEAT 2 REPEAT 1179 1217 HEAT 3 REPEAT 1354 1392 HEAT 4 REPEAT 1636 1674 HEAT 5 REPEAT 1680 1718 HEAT 6	1716 1717 NF->TS: Abolihes binding to acetylated histones	Y
15	Q08AM6	Protein VAC14 homolog	782	REPEAT 5 42 HEAT 1 REPEAT 89 126 HEAT 2 REPEAT 171 208 HEAT 3 REPEAT 212 249 HEAT 4 REPEAT 438 475 HEAT 5 REPEAT 560 598 HEAT 6	773 773 G->A: Reduces interaction with NOS1 774 774 D->A: Reduces interaction with NOS1 776 776 L->A: Reduces interaction with NOS1 777 777 D->A: Abolishes interaction with NOS1 780 782 VVL->AAA: Reduces interaction with NOS1 782 782 L->G: Reduces interaction with NOS1	N
16	O75533	Splicing factor 3B subunit 1	1304	REPEAT 529 568 HEAT 1 REPEAT 569 603 HEAT 2 REPEAT 604 641 HEAT 3 REPEAT 643 677 HEAT 4 REPEAT 680 718 HEAT 5 REPEAT 763 801 HEAT 6 REPEAT 843 881 HEAT 7 REPEAT 1010 1048 HEAT 8 REPEAT 1052 1090 HEAT 9 REPEAT 1122 1160 HEAT 10 REPEAT 1163 1201 HEAT 11	200 200 W->A: Abolishes interaction with RBM39 when associated with A-218 A-232 A-254 A-293 A-310 and A-338 218 218 W->A: Abolishes interaction with RBM39 when associated with A-200 A-232 A-254 A-293 A-310 and A-338 223 223 T->A: No effect on interaction with PPP1R8 227 227 T->A: No effect on interaction with PPP1R8 232 232 W->A: Abolishes interaction with RBM39 when associated with A-200 A-218 A-254 A-293 A-310 and A-338 235 235 T->A: No effect on interaction with PPP1R8 244 244 T->A: Slight inhibition of interaction with PPP1R8 248 248 T->A: Slight inhibition of interaction with PPP1R8 254 254 W->A: Abolishes interaction with RBM39 when associated with A-200 A-218 A-232 A-293 A-310 and A-338 257 257 T->A: No effect on interaction with PPP1R8 261 261 T->A: Slight inhibition of interaction with PPP1R8 267 267 T->A: No effect on interaction with PPP1R8 273 273 T->A: No effect on interaction with PPP1R8 278 278 T->A: No effect on interaction with PPP1R8 293 293 W->A: Abolishes interaction with RBM39 when associated with A-200 A-218 A-232 A-254 A-310 and A-338 296 296 T->A: No effect on interaction with PPP1R8 303 303 T->A: No effect on interaction with PPP1R8 310 310 W->A: Abolishes interaction with RBM39 when associated with A-200 A-218 A-232 A-254 A-293 and A-338 313 313 T->A: No effect on interaction with PPP1R8 338 338 W->A: Abolishes interaction with RBM39 when associated with A-200 A-218 A-232 A-254 A-293 and A-310 700 700 K->E: Does not affect the stability of the SF3B complex interaction with U2AF65	Y
17	Q9Y678	Coatomer subunit gamma-1	874	REPEAT 64 101 HEAT 1 REPEAT 283 320 HEAT 2 REPEAT 322 355 HEAT 3 REPEAT 356 392 HEAT 4	776 776 W->S: Loss of interaction with ZNF289/ARFGAP2	N
18	O43432	Eukaryotic translation initiation factor 4 gamma 3	1585	REPEAT 745 783 HEAT 1 REPEAT 784 831 HEAT 2 REPEAT 832 905 HEAT 3 REPEAT 906 944 HEAT 4 REPEAT 945 984 HEAT 5	756 756 R->D: Reduces binding to EIF4A when associated with D-759 and D-764 759 759 R->D: Reduces binding to EIF4A when associated with D-756 and D-764 764 764 K->D: Reduces binding to EIF4A when associated with D-756 and D-759 814 814 R->D: Reduces binding to EIF4A when associated with D-820 820 820 K->D: Reduces binding to EIF4A when associated with D-814 834 835 RK->DD: Reduces binding to IRES	Y
19	Q8WYA6	Beta-catenin-like protein 1	563	REPEAT 79 129 HEAT 1 REPEAT 134 176 HEAT 2	16 33 Missing: Nuclear and cytoplasmic localization 521 563 Missing: No change in NLS binding nor folding	N
20	Q13535	Serine/threonine-protein kinase ATR	2644	REPEAT 799 835 HEAT 1 REPEAT 1329 1365 HEAT 2	2327 2327 K->R: Abolishes kinase activity 2475 2475 D->A: Abolishes kinase activity increases sensitivity to IR and impairs translocation to nuclear foci upon DNA damage 2494 2494 D->E: Abolishes kinase activity reduces cell viability, augments sensitivity to IR and UV	N
21	Q96T76	MMS19 nucleotide excision repair protein homolog	1030	REPEAT 866 904 HEAT 1 REPEAT 908 946 HEAT 2 REPEAT 949 987 HEAT 3 REPEAT 990 1028 HEAT 4	993 993 K->R: Impairs MAGEF1-NSMCE1-mediated polyubiquitination when associated with R-1002, 1007-R-R-1008 and R-1013 1002 1002 K->R: Impairs MAGEF1-NSMCE1-mediated polyubiquitination when associated with R-993, 1007-R-R-1008 and R-1013 1007 1008 KK->RR: Impairs MAGEF1-NSMCE1-mediated polyubiquitination when associated with R-993, R-1002 and R-1013 1013 1013 K->R: Impairs MAGEF1-NSMCE1-mediated polyubiquitination when associated with R-993, R-1002 and 1007-R-R-1008	Y
22	Q96Q15	Serine/threonine-protein kinase SMG1	3661	REPEAT 1817 1852 HEAT	2335 2335 D->A: Loss of function	N
23	Q9BU89	Deoxyhypusine hydroxylase	302	REPEAT 54 80 HEAT-like PBS-type 1 REPEAT 87 113 HEAT-like PBS-type 2 REPEAT 174 200 HEAT-like PBS-type 3 REPEAT 205 231 HEAT-like PBS-type 4 REPEAT 238 264 HEAT-like PBS-type 5	56 56 H->A: Loss of deoxyhypusine monooxygenase activity 57 57 E->A: Loss of deoxyhypusine monooxygenase activity 86 86 M->A: Loss of iron-binding 86 86 M->L: No effect on iron-binding when associated with L-237 89 89 H->A: Loss of deoxyhypusine monooxygenase activity 90 90 E->A: Loss of deoxyhypusine monooxygenase activity 207 207 H->A: Loss of deoxyhypusine monooxygenase activity 208 208 E->A: Loss of deoxyhypusine monooxygenase activity 237 237 M->L: Decreased iron-binding when associated with L-86 240 240 H->A: Loss of deoxyhypusine monooxygenase activity 241 241 E->A: Loss of deoxyhypusine monooxygenase activity	Y

Details of 22 HEAT Repeats and their Mutations